PURIFICATION AND CHARACTERIZATION OF SOY COTYLEDON β-GLUCOSIDASE
نویسندگان
چکیده
منابع مشابه
Purification and characterization of an extracellular β‐glucosidase from Sporothrix schenckii
An extracellular β-glucosidase (E.C. 3.2.1.21), induced by cellulose in the mycelial form of human pathogen fungus Sporothrix schenckii, was purified to homogeneity using hydroxyapatite (HAp) adsorption chromatography in batch and Sephacryl S200-HR size exclusion chromatography. The molecular mass of the purified enzyme was estimated to be 197 kDa by size exclusion chromatography with a subunit...
متن کاملCloning, Purification, and Characterization of a Thermostable β-Glucosidase from Thermotoga thermarum DSM 5069
A 56-kDa β-glucosidase (TthBgl) derived from Thermotoga thermarum DSM 5069 was expressed and purified from Escherichia coli BL21 (DE3). The purified enzyme showed hydrolytic activity towards only pnitrophenyl-β-D-glucopyranoside among the synthetic glycosides tested. The pH maximum was 5.0, and under the conditions tested, maximal activity was at 85 oC, and pH stability occurred from 5.0 to 6.0...
متن کاملPurification and Characterization of a Ginsenoside Rb1-Hydrolyzing β-Glucosidase from Aspergillus niger KCCM 11239
Rb(1)-hydrolyzing β-glucosidase from Aspergillus niger KCCM 11239 was studied to develop a bioconversion process for minor ginsenosides. The specific activity of the purified enzyme was 46.5 times greater than that of the crude enzyme. The molecular weight of the native enzyme was estimated to be approximately 123 kDa. The optimal pH of the purified enzyme was pH 4.0, and the enzyme proved high...
متن کاملPurification and Partial Characterization of β-Glucosidase in Chayote (Sechium edule).
β-Glucosidase (EC 3.2.1.21) is a prominent member of the GH1 family of glycoside hydrolases. The properties of this β-glucosidase appear to include resistance to temperature, urea, and iodoacetamide, and it is activated by 2-ME, similar to other members. β-Glucosidase from chayote (Sechium edule) was purified by ionic-interchange chromatography and molecular exclusion chromatography. Peptides d...
متن کاملScreening and characterization of β-glucosidase production by Saccharomyces cerevisiae
Article history: Received on: 24/11/2015 Revised on: 04/12/2015 Accepted on: 23/01/2016 Available online: 28/05/2016 Beta-Glucosidases (BGS) are the group of hydrolase enzymes, involved in the degradation processes and many biological processes. Due to demand, intensive screening of BGS is required to explore the natural microbial source of BGS. The current study deals with isolation and identi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Food Biochemistry
سال: 2012
ISSN: 0145-8884
DOI: 10.1111/j.1745-4514.2011.00632.x